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MACHEREY-NAGEL 720951.46 Nucleosil 100-7 C18 HPLC 1 Column, Analytical, 125 mm Length, 4.6 mm ID, USP L1
ETB 137072
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NUCLEOSIL is widely accepted as a routine packing for various fields of chromatography.
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Product Details
| Brand Name | MACHEREY-NAGEL |
| Manufacturer | MACHEREY-NAGEL |
| Included Components | HPLC Column |
| Part Number | 720951.46 |
| Item Weight | 0.5 lbs (230 grams) |
Who Should Buy?
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Analytical Chemists
Ideal for chemists requiring high precision and reliable results in analytical testing and method development.
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Research Laboratories
Excellent for laboratories focused on chromatography applications needing quality and consistency with C18 column technology.
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Pharmaceutical Testing
Suitable for pharmaceutical companies conducting analysis of compounds ensuring accurate drug formulation and quality control.
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Non-Analytical Users
Not suitable for users who do not require high-performance liquid chromatography in their processes.
Product Description
MACHEREY-NAGEL 720951.46 Nucleosil 100-7 C18 HPLC 1 Column, Analytical, 125 mm Length, 4.6 mm ID, USP L1
Customer Questions & Answers
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Question:
What is the primary use of the MACHEREY-NAGEL 720951.46 Nucleosil 100-7 C18 HPLC column?
Answer: The MACHEREY-NAGEL 720951.46 Nucleosil 100-7 C18 HPLC column is primarily used for high-performance liquid chromatography (HPLC) applications in analytical chemistry. It efficiently separates a wide range of compounds, including small molecules and complex mixtures, due to its C18 stationary phase. This column is ideal for purifying compounds in pharmaceuticals, environmental monitoring, and food safety testing. Utilizing this HPLC column ensures high resolution and sensitivity in your analyses, making it a reliable choice for laboratories. -
Question:
What are the dimensions and specifications of the HPLC column?
Answer: The MACHEREY-NAGEL 720951.46 Nucleosil HPLC column has a length of 125 mm and an inner diameter (ID) of 4.6 mm. It features a USP classification of L1, indicating it has a C18 phase which is suitable for a wide variety of non-polar to moderately polar compounds. These specifications contribute to the column's versatility and compatibility with various HPLC systems, allowing for effective and efficient separation in numerous applications across analytical fields. -
Question:
How does the C18 stationary phase benefit my HPLC analysis?
Answer: The C18 stationary phase in the MACHEREY-NAGEL column allows for optimal retention and separation of hydrophobic compounds. Its non-polar nature interacts effectively with non-polar analytes through van der Waals forces, resulting in improved peak shape and resolution during HPLC analysis. This is particularly beneficial when working with complex mixtures in pharmaceutical and biochemical applications, where precise identification and quantification of compounds are critical for research and quality control. -
Question:
Can the MACHEREY-NAGEL column be used for both reverse phase and normal phase chromatography?
Answer: The MACHEREY-NAGEL 720951.46 Nucleosil 100-7 column is specifically designed for reverse phase chromatography. It utilizes a C18 stationary phase, making it unsuitable for normal phase applications which typically require silica-based stationary phases for polar compounds. For optimal results in reverse phase applications, this column excels in separating non-polar to moderately polar compounds such as pharmaceuticals, environmental samples, and biomolecules. -
Question:
What types of solvents can be used with this HPLC column?
Answer: This HPLC column is compatible with a wide range of solvents, including water, methanol, acetonitrile, and ethanol, among others. The choice of solvent can greatly affect the separation efficiency and resolution of your target compounds. For most applications, a binary or ternary solvent system is employed to optimize peak shape and enhance retention times, making it crucial to select the right solvent combination for your specific analysis. -
Question:
What is the recommended flow rate for the MACHEREY-NAGEL HPLC column?
Answer: The recommended flow rate for the MACHEREY-NAGEL 720951.46 Nucleosil 100-7 HPLC column typically ranges between 0.5 to 1.5 mL/min, depending on the specific application and analyte characteristics. Adjusting the flow rate can affect retention times, peak resolution, and analysis sensitivity. It's advisable to conduct preliminary experiments to find the optimal flow rate for your method, ensuring high-quality results in your HPLC analyses. -
Question:
How do I store the MACHEREY-NAGEL 720951.46 column to maintain its performance?
Answer: To maintain the performance of the MACHEREY-NAGEL 720951.46 column, store it in a temperature-controlled environment. Recommended conditions are typically around room temperature, and it should be kept away from direct sunlight. It’s essential to store the column in a suitable solvent that is compatible with its stationary phase to avoid degradation or contamination. Proper storage extends the life of the column and ensures consistent analytical results during use. -
Question:
What sample types can I analyze with this HPLC column?
Answer: The MACHEREY-NAGEL 720951.46 column is ideal for analyzing various sample types, including pharmaceutical compounds, environmental samples, food and beverage products, and biological fluids. It effectively separates polar to non-polar compounds, making it versatile for different analytical needs. Researchers and quality control laboratories commonly utilize this column for applications that require high precision and accuracy in compound identification and quantification. -
Question:
Are there any contaminants that should be avoided when using this column?
Answer: When using the MACHEREY-NAGEL 720951.46 column, it's important to avoid introducing strongly adsorptive or reactive contaminants that might damage the C18 stationary phase. Avoid using buffered mobile phases with high salt concentrations or solvents known to cause phase collapse. Additionally, certain harsh organic solvents can affect the column's performance. Ensuring that the sample is properly filtered and within the column's specifications will preserve its integrity and longevity. -
Question:
Where can I buy MACHEREY-NAGEL 720951.46 Nucleosil 100-7 C18 HPLC Column in Ethiopia?
Answer: You can buy the MACHEREY-NAGEL 720951.46 Nucleosil 100-7 C18 HPLC column on Ubuy, a reliable online platform that specializes in a wide range of laboratory products and equipment. Ubuy offers convenient access to this premium HPLC column, allowing you to enhance your analytical capabilities with high-quality chromatography solutions directly from their website.
MACHEREY-NAGEL LC Columns & Column Supplies Editorial Review
The MACHEREY-NAGEL 720951.46 Nucleosil 100-7 C18 HPLC column offers precise analytical performance with a length of 125 mm and an inner diameter of 4.6 mm, making it suitable for various HPLC applications. This column is manufactured by MACHEREY-NAGEL, known for its reliable and high-quality laboratory products. The item weight of 0.5 lbs indicates its compact yet robust design. Reviewers appreciate its efficiency in providing accurate separation results, making it a valuable component for analytical chemists. Its compatibility with various solvents enhances its usability in different laboratory settings, creating a versatile solution for sample analysis.
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Pros
- High precision for analytical applications
- Compatible with various solvents
- Reliable performance for lab tests
- Compact and lightweight design
- Manufactured by a reputable brand
Cons
- Limited user feedback available
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ETB 137072
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Features & Benefits
- High purity and uniform SiO2 structure for reliable results.
- End capped with a pore size of 100 Å for optimal performance.
- Particle size of 5 µm enhances separation efficiency.
- Utilizes hydrophobic interactions for effective separation mechanism.
- Versatile application in different chromatography fields.
- One of the first spherical silicas developed for HPLC.
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