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Accuris PR2000-H-500 qMax Green, High Rox qPCR Mix, 500 Reactions
84% of respondents would recommend this to a friend
ETB 59611
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Superior sensitivity and fast cycling with exceptional results.
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What Stands Out
Product Details
| Button Quantity | 3 |
| Power Source | Battery Powered |
| Range | 10.0 meters |
| Color | Green |
| Style Name | Modern |
| Brand | Accuris |
| Manufacturer | Accuris |
| Mfr Part Number | PR2000-H-500 |
| Number of Buttons | 3 |
| Style | Modern |
| Item Weight | 1 lbs (450 grams) |
Who Should Buy?
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Research Laboratories
Ideal for academic labs conducting gene expression studies requiring reliable and high-throughput qPCR performance.
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Clinical Applications
Suitable for diagnostic labs needing precise quantification in various clinical research applications, ensuring reproducibility.
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Environmental Testing
Effective for monitoring pathogens or contaminants in environmental samples, leveraging high sensitivity in diverse conditions.
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Beginners
Not preferred for those new to qPCR, as it may require advanced knowledge for optimal use and setup.
Product Description
Accuris PR2000-H-500 qMax Green, High Rox qPCR Mix, 500 Reactions
Customer Questions & Answers
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Question:
What is the PR2000-H-500 qMax Green, High Rox qPCR Mix?
Answer: The PR2000-H-500 qMax Green, High Rox qPCR Mix is a high-performance polymerase mix designed for quantitative polymerase chain reaction (qPCR) applications. This mix incorporates a unique green dye and high Rox reference dye to ensure optimal signal detection and enable quantification in various biological experiments. It's specifically formulated to enhance the efficiency of PCR, providing consistent and reliable results. Researchers often use this product in gene expression studies, pathogen detection, and genotyping applications. -
Question:
What are the benefits of using High Rox qPCR Mix?
Answer: High Rox qPCR Mix offers several notable benefits, including improved assay sensitivity and reproducibility. The Rox dye serves as a passive reference, which normalizes signal fluctuations during PCR, leading to enhanced accuracy in quantifying nucleic acids. This is especially beneficial in multiplex assays, where different targets are analyzed in the same reaction. Users frequently report better results in experiments requiring precise quantification, such as studying low-abundance transcripts or exploring rare genetic variants. -
Question:
How do you use the PR2000-H-500 qMax Green Mix in qPCR?
Answer: Using the PR2000-H-500 qMax Green Mix in qPCR involves a simple protocol. First, prepare your reaction mix by combining the qPCR mix with primers, probes, and template DNA. The recommended final concentration of the mix is generally around 1X, but always consult the specific protocol provided by your laboratory. Once mixed, transfer the reaction to a qPCR plate, seal it, and place it in a qPCR machine. This mix is versatile and can be applied in various experiments, from basic research to clinical diagnostics. -
Question:
Is the PR2000-H-500 qMax Green Mix suitable for multiplex qPCR?
Answer: Yes, the PR2000-H-500 qMax Green Mix is suitable for multiplex qPCR applications. The high Rox stabilization allows for precise quantification even when multiple target sequences are amplified in the same reaction. Researchers commonly utilize multiplexing to conserve reagents and reduce sample handling time, making this mix an excellent choice for labs aiming to streamline their workflows. Additionally, users have found success in experiments where simultaneous detection of various pathogens is required. -
Question:
Can I store the PR2000-H-500 qMax Green Mix? If so, how?
Answer: The PR2000-H-500 qMax Green Mix should be stored at -20°C for long-term storage to maintain its activity and effectiveness. It's important to avoid repeated freeze-thaw cycles as they can degrade the components over time. For optimal performance, aliquoting the mix into smaller portions can help preserve its integrity. This practice is particularly useful for laboratories that frequently use this mix, allowing them to thaw only what is needed for immediate use. -
Question:
What types of samples can be analyzed using this qPCR mix?
Answer: The PR2000-H-500 qMax Green Mix is compatible with various sample types, including cDNA, genomic DNA, and RNA samples after reverse transcription. Whether you're working with clinical samples, environmental samples, or cultured cells, this mix can effectively amplify nucleic acid targets. It's versatile, making it ideal for applications ranging from pathogen detection to gene expression analysis in different research environments, enhancing the flexibility of your experimental design. -
Question:
Are there any specific primers or probes recommended for use with this mix?
Answer: While there are no strict primer or probe recommendations exclusively for the PR2000-H-500 qMax Green Mix, it’s crucial to design them specifically for your target sequences considering factors such as annealing temperature and GC content. Optimized primers and probes enhance the efficiency of your qPCR reaction, and using this mix allows flexibility in designing your assays. Researchers often test various primer sets to achieve optimal results tailored to their specific experimental conditions. -
Question:
What is the shelf life of PR2000-H-500 qMax Green Mix?
Answer: The shelf life of the PR2000-H-500 qMax Green Mix is typically indicated on the product packaging or data sheet. Generally, when stored properly at -20°C, users can expect a shelf life of several months to over a year without significant loss of performance. Always check the expiration date and perform control experiments to ensure the mix is still effective, especially if you have had it in storage for an extended period. -
Question:
Can this qPCR mix be used in quantitative and qualitative assays?
Answer: Yes, the PR2000-H-500 qMax Green Mix can be utilized in both quantitative (qPCR) and qualitative PCR assays. In quantitative assays, the concentration of template DNA can be accurately measured, while in qualitative assays, the presence or absence of a specific target can be determined. This versatility makes it suitable for a broad range of applications, from basic research to diagnostic testing, providing researchers with a reliable tool for their molecular biology needs. -
Question:
Where can I buy PR2000-H-500 qMax Green, High Rox qPCR Mix, 500 Reactions?
Answer: You can buy the PR2000-H-500 qMax Green, High Rox qPCR Mix from Ubuy. They offer this product online, ensuring that you're able to find it with ease. Ubuy provides a straightforward purchasing experience, making it simple for you to acquire this high-quality qPCR mix to support your research and experiments.
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ETB 59611
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Features & Benefits
- Ready-to-use 2X master mix for convenience.
- Engineered for high sensitivity and fast cycling.
- Ideal for low copy number and difficult template sequences.
- Early Ct values for quicker detection.
- Includes Accuris Hot Start Taq Polymerase for accuracy.
- Proprietary dye shows higher fluorescence and lower PCR inhibition.
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